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  • Title: A microplate enzyme-linked immunospecific assay (ELISA) detecting unbound anti-platelet antibodies.
    Author: Fabris F, Casonato A, Zanchetta R, Cortellazzo S, Busolo F, Girolami A.
    Journal: Folia Haematol Int Mag Klin Morphol Blutforsch; 1984; 111(1):66-71. PubMed ID: 6202601.
    Abstract:
    A microplate immune-enzymatic method was developed for detecting serum antiplatelet antibodies. The method involves the use of antigen-coated platelets and alkaline phosphatase-labeled antihuman immunoglobulin. Linear correlation was obtained between the titer of platelet antibodies and substrate conversion. Twenty-four patients with immune thrombocytopenia and 40 normal controls were studied. Eighteen patients and one control were positive. Therefore, sensitivity and specificity were 75% and 97% respectively. ELISA also was found to be more sensitive than the indirect antiglobulin consumption assay (ACA) and appears to be a practical and easy method for routine evaluation of antiplatelet antibodies.
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