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  • Title: Analysis of plasmid DNA synthesis by double tracer method--differential effects of rifampicin and chloramphenicol on the replication of pSC101 and ColEl-amp in Escherichia coli K-12.
    Author: Hasunuma K, Furukawa K.
    Journal: Radioisotopes; 1983 Dec; 32(12):609-16. PubMed ID: 6203141.
    Abstract:
    An Escherichia coli strain, CR34 , harboring both pSC101 and ColEl -amp plasmids was exposed to media containing rifampicin (100 micrograms/ml) and/or chloramphenicol (180 micrograms/ml) and the cells were labeled for 20 min with 3H-thymine at 3, 25 and 50 min after exposure to drug(s). The plasmid DNA synthesis was assayed by DNA-DNA hybridization with 14C-labeled pSC 134 DNA as internal marker. In the presence of rifampicin, the replication of pSC 101 was from 57 to 104% that in its absence, and that of ColEl -amp was from 17 to 26%. The DNA replication of pSC 101 after addition of chloramphenicol was reduced to 35 to 75%, and that of ColEl -amp was reduced to 39% and then restored to 92%. This restoration was not observed in the presence of rifampicin.
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