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  • Title: Peroxidase immunocytochemistry for the detection of specific anti-hapten (penicilloyl) antibody producing cells in the spleen, after injection of a hapten-carrier conjugate.
    Author: van Rooijen N, Kors N, Boorsma DM, de Haan P, van Nieuwmegen R.
    Journal: Immunol Commun; 1984; 13(1):29-34. PubMed ID: 6203828.
    Abstract:
    Cells producing specific antibodies against the hapten penicilloyl (Pen) are demonstrated in tissue sections of the spleen, using peroxidase immunocytochemistry. Horseradish peroxidase-human serum albumin-penicilloyl (HRP-HSA-Pen) conjugate, prepared by coupling Pen to HRP-HSA conjugate, was used for detection of anti-Pen producing cells in the spleen after intravenous injection of bovine gamma globulin-penicilloyl (BGG-Pen) conjugate. HRP-HSA conjugate was used as a control to exclude the possibility that positive cells contained antibody against a common determinant in both HSA and BGG. The method may be applied for the detection of cells producing antibodies against other haptens as well.
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