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  • Title: Production and characterization of monoclonal antibodies to peripheral and central nervous system myelin.
    Author: Miller SL, Pleasure D, Herlyn M, Atkinson B, Ernst C, Tachovsky TG, Baird L.
    Journal: J Neurochem; 1984 Aug; 43(2):394-400. PubMed ID: 6204014.
    Abstract:
    Monoclonal antibodies against P0, myelin basic protein, or myelin-associated glycoprotein were generated by fusing mouse myeloma cells with spleen cells from BALB/c mice immunized with central and peripheral nervous system myelin proteins. The antibodies secreted were either IgG, IgM, or IgA. Clone C6B5 (iso-type IgM) secreted antibody(ies) that bound to both myelin basic protein and myelin-associated glycoprotein, although binding of antibody to myelin basic protein as detected by the immunoblot technique appeared to be much less than to the myelin-associated glycoprotein. Antibodies were characterized in solid-phase radioimmunoassay for their species cross-reaction, and histologically for the specificity of binding to myelin in central and peripheral nervous system tissues. These monoclonal reagents should prove valuable in studying CSF and myelin-producing cells, since in both cases the concentration of myelin proteins is low.
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