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  • Title: The small nuclear ribonucleoproteins that react with anti-Sm and anti-RNP antibodies.
    Author: Martí A, Matsuda M, Sihag RK, Eliceiri GL.
    Journal: Biochem Biophys Res Commun; 1984 Aug 30; 123(1):345-51. PubMed ID: 6206862.
    Abstract:
    We have examined the polypeptide pattern of small nuclear ribonucleoprotein particles that react with monoclonal anti-Sm antibodies or polyclonal anti-(U1)RNP antibodies. The fresh nuclear extracts analyzed were prepared from human cells that had been pulse-chased with a mixture of 15 3H-labeled amino acids. In contrast to previous reports in the literature, the apparent molecular weights of the major polypeptides that remained in the 1 M NaCl-washed ribonucleoprotein-antibody complexes were approximately 80000, 55000, 28000, 25000, 14000 and 9000, when probed with monoclonal anti-Sm antibodies, and about 69000, 58000 and 35000, when polyclonal anti-(U1)RNP antibodies were used.
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