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  • Title: Frequencies of phosphorylcholine-specific and T15-associated 10/13 idiotope-positive B cells within lipopolysaccharide-reactive B cells of adult BALB/c mice.
    Author: Lévy M.
    Journal: Eur J Immunol; 1984 Sep; 14(9):864-8. PubMed ID: 6207032.
    Abstract:
    The frequencies of lipopolysaccharide-reactive B lymphocytes in adult BALB/c spleen cells were determined by limiting dilution experiments to be between 1 in 50 and 1 in 100. Within this reactive population, the frequencies of B lymphocytes specific for phosphorylcholine (PC) and/or binding a TEPC 15-associated idiotope-specific monoclonal antibody (10/13) were found to be between 1 in 50 and 1 in 500. Binding of these antibodies to immobilized PC or to monoclonal idiotope-specific antibodies could be inhibited by soluble PC in a solid-phase radioimmunoassay. Around half the PC-specific B cell clones were found to also bind 10/13 idiotope-specific monoclonal antibody, while the rest were either PC specific, but idiotope-negative or idiotope-positive, and unable to bind immobilized PC.
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