These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Fluorouracil synchronization of human bone marrow cultures. In vitro induction of high resolution R-banding by simultaneous exposure to 5-bromodeoxyuridine/Hoechst 33258.
    Author: Rønne M.
    Journal: Anticancer Res; 1984; 4(4-5):279-81. PubMed ID: 6207765.
    Abstract:
    A simple R-banding technique, based on a fluorouracil S-phase block, released by exposure to 5-bromodeoxyuridine and Hoechst 33258 is applied to human bone marrow cells. It is both simple and reliable, plus ensuring an index corresponding to that of the methotrexate method. Due to Hoechst 33258 exposure prior to harvest, chromosome contraction is partly inhibited. Well defined R-banding in elongated prometaphase chromosomes is observed both after traditional Giemsa and FPG-staining.
    [Abstract] [Full Text] [Related] [New Search]