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  • Title: High-performance liquid chromatography of proteins: purification of alpha-fetoprotein from fetal calf serum.
    Author: Wong LT, Hsia JC.
    Journal: J Chromatogr; 1984 Sep 14; 310(1):19-29. PubMed ID: 6209297.
    Abstract:
    High-performance liquid chromatography was utilized for the purification of bovine alpha-fetoprotein (BAFP) from fetal calf serum (FCS). An initial step in the purification involved absorption of charcoal delipidated FCS on Cibacron Blue F3GA gel. The Cibacron Blue pre-purified FCS was then chromatographed on a Polyanion SI weak anion-exchange column. The BAFP isolated had a purity of greater than 93% with an overall yield of 48% from FCS. The procedure was applicable for semi-preparative scale purification of BAFP.
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