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  • Title: Intercellular NOR-Ag variability in man. I. Technical improvements and marker acrocentric chromosomes.
    Author: Sozansky OA, Zakharov AF, Benjush VA.
    Journal: Hum Genet; 1984; 68(4):299-302. PubMed ID: 6210238.
    Abstract:
    A new modification of the Ag I technique has been developed using human cultured blood lymphocytes, which involves ultra-violet irradiation of chromosome preparations during incubation in AgNO3. This technique enables detection in a short incubation time all NORs capable of being stained with silver. A peculiar morphological change in Ag-stainable NORs during the incubation is described, which can be used as a criterion of the completion of Ag staining. With the refined Ag-staining procedure, acrocentric marker chromosomes were studied which showed one or two satellite stalks within the same individual. Ag staining was highly coincident with this variability.
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