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  • Title: Reversed-phase paired-ion high-performance liquid chromatographic method for the separation and quantification of multiple bleomycin congeners.
    Author: Klett RP, Chovan JP, Danse IH.
    Journal: J Chromatogr; 1984 Oct 12; 310(2):361-71. PubMed ID: 6210296.
    Abstract:
    A rapid, linear gradient chromatographic technique for separating and quantifying copper(II)-chelated bleomycin congeners is described. This method is also capable of separating divalent from trivalent metal chelates; determining the purity of many chemically modified bleomycins; and assaying bleomycin hydrolase activity on complex mixtures. Quantification at 280 nm is sensitive to 50 pmol and is linear at least up to 10 nmol per injection.
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