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  • Title: Determination of sodium phenobarbital in animal chow by high-pressure liquid chromatography.
    Author: Bowman MC, Rushing LG.
    Journal: J Chromatogr Sci; 1978 Jan; 16(1):23-7. PubMed ID: 621271.
    Abstract:
    An analytical procedure is described for determining residues of sodium phenobarbital in animal chow at levels as low as 0.14 ppm. The methanol extract is subjected to a liquid-liquid cleanup at pH 13 and 1, further cleaned up on a silica gel column and assayed by high-pressure liquid chromatography by using an ultraviolet absorption detector at 210 nm. Data concerning extraction efficiency, partition values and stability of the chemical in animal chow are also presented.
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