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  • Title: Reconstitution of stellacyanin as a case of direct Cu(I) transfer between yeast copper thionein and 'blue' copper apoprotein.
    Author: Hartmann HJ, Morpurgo L, Desideri A, Rotilio G, Weser U.
    Journal: FEBS Lett; 1983 Feb 07; 152(1):94-6. PubMed ID: 6220920.
    Abstract:
    It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu-thionein, without any detectable unspecific Cu(II) intermediate, both aerobically and anaerobically.
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