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  • Title: [Purification of epidermal G2-chalone using immunoaffinity chromatography].
    Author: Ketlinskiĭ SA, Simbirtsev AS.
    Journal: Biull Eksp Biol Med; 1983 Aug; 96(8):71-3. PubMed ID: 6224519.
    Abstract:
    Epidermal G2-chalone was purified from rat skin extract by immunoaffinity chromatography with the use of mono-specific antibodies coupled to CNBr-activated sepharose 4B. During SDS PAAG electrophoresis, it produced a single band with a molecular weight of 13000 dalton. G2-chalone purified in this way had a 60% antimitotic inhibitory effect on external ear epidermocytes in mice in a dose of 2 micrograms/g bw but had no effect on the mitosis in the sebaceous glands.
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