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  • Title: Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa.
    Author: Smith DD, Coggins JR.
    Journal: Biochem J; 1983 Aug 01; 213(2):405-15. PubMed ID: 6225423.
    Abstract:
    Limited proteolysis of the arom enzyme complex of Neurospora crassa by trypsin or subtilisin yielded a stable fragment of Mr 68000. This fragment, which was purified by two-dimensional polyacrylamide-gel electrophoresis, was shown by activity staining to contain the shikimate dehydrogenase active site, and by substrate labelling with 3-dehydroquinate and NaB3H4 to contain the 3-dehydroquinase active site. The fragment thus constitutes a bifunctional domain containing the two enzymic activities that are known, from genetic evidence, to be located adjacently at the C-terminal end of the pentafunctional arom polypeptide.
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