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  • Title: Identification of macrophages and of a T-lymphocytes subpopulation in normal human lymphnode by histochemical demonstration of alpha-naphthyl-acetate-esterasic activity.
    Author: Manara GC, Scandroglio R, De Panfilis G, Allegra F.
    Journal: Acta Biomed Ateneo Parmense; 1983; 54(3):237-45. PubMed ID: 6228089.
    Abstract:
    The cell population of normal human lymphnode was studied by a histochemical technique showing alpha-naphthyl-acetate-esterasic activity. Two distinct cell populations were evidenced: a) macrophagic cells, bearing a diffused ANAE cytoplasmic positivity, most of which were in the subcapsular area, whereas a minority of them was found within the lymphatic nodules and the paracortical area; b) lymphocytic cells, bearing on ANAE positive cytoplasmic spot, most of which were in the paracortical area, whereas a minority of them was found within the lymphatic nodules. A discussion of the results we got, as compared with those which are reported by literature, confirms what is already well-known about the cell colonization of the various areas in the lymphnode. Besides it gives a chance to identify lymphocytes bearing an ANAE positive cytoplasmic spot with T lymphocytes belonging to the "helper-inducer" subclass.
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