These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The murine lymphocyte receptor for IgE. I. Isolation and characterization of the murine B cell Fc epsilon receptor and comparison with Fc epsilon receptors from rat and human.
    Author: Conrad DH, Peterson LH.
    Journal: J Immunol; 1984 Feb; 132(2):796-803. PubMed ID: 6228601.
    Abstract:
    The Fc receptor for IgE (Fc epsilon R) on murine B lymphocytes was studied by using BALB/c mice infected 12 to 18 days previously with Nippostrongylus brasiliensis. B cells were enriched in the Sephadex G-10-passed lymphocytes by treating with anti-Thy-1.2 and complement (C). After stripping any cytophilic Ig with low pH, the B cells were 125I surface labeled; subsequently the membranes were solubilized with nonionic detergent, and putative Fc epsilon R components were allowed to bind to IgE-coated adsorbents. Bound radiolabel was eluted with low pH, and when examined by SDS-PAGE, was found to consist primarily of a relatively broad band centered at 49,000 m.w. (49K). Fluid-phase IgE could prevent the binding of the 49K component to the IgE solid-phase adsorbents. Rebinding studies further indicated that the 49K component exhibited a specificity for IgE, thus confirming that the 49K component was the murine B lymphocyte Fc receptor for IgE (Fc epsilon R). Some rebinding to rabbit IgG was observed, and by using 2.4G2, the monoclonal anti-Fc gamma 2b receptor (Fc gamma 2bR) antibody to isolate the IgG2b receptor, a clear distinction between the FC gamma 2bR and the 49K IgE receptor was demonstrated by SDS-PAGE analysis. Rabbit IgG was thus found to interact with both the 49K Fc epsilon R and the 59K FC gamma 2bR. The murine B lymphocyte Fc epsilon R was compared with the human B cell Fc epsilon R from the RPMI 8866 cell line and with the high affinity Fc epsilon R on rat basophilic leukemia cells by one- and two-dimensional gel analyses. The lymphocyte Fc epsilon R from mouse and human was found to be quite similar with respect to m.w. (45 to 50K) and isoelectric point (pI 4.5 to 5.0), whereas the basophil Fc epsilon R differed in both aspects.
    [Abstract] [Full Text] [Related] [New Search]