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  • Title: [Toxinogenic vomitoxin-producing fusaria isolated from wheat].
    Author: Veselá D, Veselý D.
    Journal: Vet Med (Praha); 1983 Nov; 28(11):687-92. PubMed ID: 6230791.
    Abstract:
    Out of the 25 strains of the genus Fusarium isolated from wheat visibly infested by moulds, three strains of F. graminearum were found to produce vomitoxin and zearalenone; the respective amounts of these toxins per 1 kg of wheat were 70, 100 and 600 mg vomitoxin, and 600, 400 and 250 mg zearalenone. The production of vomitoxin showed a marked decrease when the cultures were re-inoculated. An analytic determination of vomitoxin in cereals was worked out by the method of high-pressure liquid chromatography with a limit sensitivity of 20 micrograms vomitoxin per kg and by the method of thin-layer chromatography with a limit sensitivity of 100 micrograms vomitoxin per kg. The embryotoxicity of vomitoxin measured in chick embryos incubated for 40 hours was in the order of 1 microgram per embryo.
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