These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Glycosyl transferase activity in the brush border membrane of Hymenolepis diminuta. Author: Pappas PW. Journal: J Parasitol; 1983 Dec; 69(6):1055-9. PubMed ID: 6232361. Abstract: The isolated brush border membrane of Hymenolepis diminuta incorporates radiolabeled glucose when incubated in the presence of uridine diphospho(UDP)-D-(6-3H)glucose. The pH optimum for incorporation was 7.0 to 7.2 regardless of the buffer used. Transferase activity was maximal in 200 mM Tris buffer; 100 mM phosphate buffer inhibited significantly the incorporation of radiolabeled glucose, whereas 50 mM Tris-maleate and 100 mM PIPES resulted in moderate inhibition of activity. Incorporation of labeled glucose was not inhibited by low concentrations (0.01%) of Triton X-100, but activity was inhibited 50% by 0.25% Triton X-100. Addition of divalent cations to the brush border membrane preparation did not activate transferase activity, but addition of chelating agents (i.e., EDTA or EGTA) inhibited transferase activity nearly 90%. Incorporation of labeled glucose was inhibited by UDP, guanosine diphosphate (GDP), UDP- and GDP-activated monosaccharides, and monosaccharides, indicating that the transferase activity lacked substrate specificity.[Abstract] [Full Text] [Related] [New Search]