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  • Title: Target size analysis of rhodopsin in retinal rod disk membranes.
    Author: Hughes SM, Harper G, Brand MD.
    Journal: Biochem Biophys Res Commun; 1984 Jul 18; 122(1):56-61. PubMed ID: 6234896.
    Abstract:
    Radiation inactivation of rhodopsin in situ using high-energy electrons gave a value for Mr of 20,200 by spectral assay, but 47,100 by assay of rhodopsin regeneration from opsin and 11-cis-retinal (sequence Mr = 38,840). No light/dark differences were seen. We conclude: (a) radiation inactivation measures the size of the functional unit, and the single hit hypothesis does not hold in our experiments; (b) 500 nm absorbance requires only about half the rhodopsin molecule to be intact, but reconstitution of rhodopsin from opsin requires the whole molecule; (c) we find no evidence for functional interactions between rhodopsin monomers in darkness or light.
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