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  • Title: Rapid thin layer chromatographic method for determination of zearalenone and zearalenol in grains and animal feeds.
    Author: Swanson SP, Corley RA, White DG, Buck WB.
    Journal: J Assoc Off Anal Chem; 1984; 67(3):580-2. PubMed ID: 6235206.
    Abstract:
    A rapid and inexpensive method has been developed for the analysis of zearalenone and zearalenol in grains and animal feeds. The method involves extraction with 75% methanol, precipitation of pigments with lead acetate, and defatting with petroleum ether. The mycotoxins are subsequently partitioned into toluene-ethyl acetate, chromatographed on high performance thin layer chromatographic plates, and detected after treatment with Fast Violet B salt solution. Sensitivity of the method is better than 80 ng/g for zearalenone and 200 ng/g for zearalenol. Ten samples can be completed in less than 2 h. The method is applicable for zearalenone in corn, wheat, barley, millet, and swine feeds.
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