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  • Title: A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.
    Author: Lang L, Kornfeld S.
    Journal: Anal Biochem; 1984 Jul; 140(1):264-9. PubMed ID: 6237594.
    Abstract:
    A simplified procedure for the synthesis of [beta-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commercially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.
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