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  • Title: Construction and properties of a ribosome-binding site mutation in gene E of phi X174 bacteriophage.
    Author: Gillam S, Astell CR, Jahnke P, Hutchison CA, Smith M.
    Journal: J Virol; 1984 Dec; 52(3):892-6. PubMed ID: 6238174.
    Abstract:
    Oligodeoxyribonucleotide mutagenesis has been used to produce a G----A mutation at nucleotide 557 of the phi X174 genome. This changes the ribosome-binding sequence GAGG of gene E to GAAG without affecting the amino acid, glutamine, encoded by the overlapping gene D. The phi X174rb(E)557 mutant does not lyse infected Escherichia coli C and therefore results in the accumulation of a large number intracellular mature phage particles. Thus, the mutation inactivates production of the gene E lytic product, presumably by blocking translation of gene E, without affecting other phage functions.
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