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  • Title: A direct, rapid, radioassay for adenosine diphosphatase.
    Author: Smith G, Smith GD, Peters TJ.
    Journal: Clin Chim Acta; 1980 Feb 28; 101(2-3):287-91. PubMed ID: 6244124.
    Abstract:
    Radiolabelled adenosine diphosphate [beta 32Pi]ADP was synthesized from radioactive orthophosphate and adenosine-5'-monophosphate with dicyclohexylcarbodiimide. The purified radioactive adenosine diphosphate was used as the substrate in a rapid, direct, one-step assay for adenosine diphosphatase in human neutrophilic leukocyte homogenates. Certain kinetic properties of the enzyme activity have been determined.
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