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  • Title: Influence of inorganic phosphate on the activity determination of isoenzymes of alkaline phosphatase in various buffer systems.
    Author: Jung K, Pergande M.
    Journal: Clin Chim Acta; 1980 Mar 28; 102(2-3):215-9. PubMed ID: 6245814.
    Abstract:
    The inhibitory effect of inorganic phosphate on the activity determination of isoenzymes of alkaline phosphatase (AP) in diethanolamine (DEA), glycine and 2-amino-2-methyl-1,3-propandiol (AMPD) buffer was studied. This effect depends on the buffer used and isoenzyme investigated. Especially the placental isoenzyme is inhibited; the inhibitory effect in DEA buffer is stronger than in the other buffers used. The requirement of purity for 4-nitrophenylphosphate with respect to its content of inorganic phosphate and conclusions for using control sera enriched with AP isoenzymes are discussed.
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