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  • Title: Isolation of the chicken thymidine kinase gene by plasmid rescue.
    Author: Perucho M, Hanahan D, Lipsich L, Wigler M.
    Journal: Nature; 1980 May 22; 285(5762):207-10. PubMed ID: 6246445.
    Abstract:
    We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.
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