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  • Title: Reconstitution of the Na+K+ pump of Ehrlich ascites tumor and enhancement of efficiency by quercetin.
    Author: Spector M, O'Neal S, Racker E.
    Journal: J Biol Chem; 1980 Jun 25; 255(12):5504-7. PubMed ID: 6247335.
    Abstract:
    Plasma membranes from Ehrlich ascites tumor cells were solubilized by octylglucoside in the presence of phospholipids. The Na+K+-ATPase was purified from this extract by adsorption and elution from thio-Seph-arose 4B. The enzyme (specific activity, 7 mumoles of ATP hydrolyzed min-1 mg of protein -1) was reconstituted into liposomes by the octyglucoside dilution procedure. An ATP-dependent Na+ influx with low efficiency was observed. On addition of appropriate amounts of quercetin, the Na+ flux/ATP hydrolysis ratio was increased from 0.4 to 1.4.
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