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  • Title: A modified paper-binding procedure for the assay of nucleus-associated protein phosphokinases.
    Author: Goueli SA, Slungaard R, Wilson MJ, Ahmed K.
    Journal: J Pharmacol Methods; 1980 May; 3(3):235-42. PubMed ID: 6248692.
    Abstract:
    Previously existing paper-binding assay procedures gave results with large variations when employed for the measurement of nucleus-associated protein phosphokinase activities. However, a modified method, utilizing the binding of 32P-labeled phosphoprotein substrates to paper and employing washing procedures in 20% trichloroacetic acid at 60 degrees to 70 degrees C, gave highly reproducible results. This modified procedure was satisfactory with either chromatin or a nonhistone protein fraction derived therefrom as a source of enzyme, and dephosphophosvitin, lysine-rich histones, or casein as phosphoprotein substrates.
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