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  • Title: Electron microscopic identification and subsequent isolation of a rotavirus from a dog with fatal neonatal diarrhea.
    Author: England JJ, Poston RP.
    Journal: Am J Vet Res; 1980 May; 41(5):782-3. PubMed ID: 6250431.
    Abstract:
    Negative contrast electron microscopic examination of intestinal contents from a 3-day-old pup with fatal diarrhea exhibited numerous rotavirus-like particles. Rotavirus was subsequently recovered, using MDCK cells, and was carried through 11 sub-passages; rotavirus-like particles were identified in each passage by negative contrast electron microscopic examination. Experimental inoculation of two 6-month-old Beagle dogs with purified intestinal contents did not result in clinical signs or virus shedding.
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