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  • Title: Transcription and in vitro processing of yeast 5 S rRNA.
    Author: Tekamp PA, Garcea RL, Rutter WJ.
    Journal: J Biol Chem; 1980 Oct 10; 255(19):9501-6. PubMed ID: 6251092.
    Abstract:
    A method is described for the isolation of a yeast chromatin fraction highly enriched in ribosomal DNA sequences. In the presence of exogenous yeast RNA polymerase III, this purified chromatin actively synthesizes a set of 5 S ribosomal RNAs all of which have 5'-sequences identical with mature 5 S RNA but which end with a variable number (up to 10) of additional residues at the 3'-terminus. These extra nucleotides are precisely removed by a processing nuclease found in the chromatin supernatant fraction.
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