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  • Title: [Diagnosis of congenital deficiency of alpha 1-antitrypsin by the immune peroxidase technique (author's transl)].
    Author: Totović V, Kron I.
    Journal: Leber Magen Darm; 1980 Apr; 10(2):91-8. PubMed ID: 6251326.
    Abstract:
    10 cases of congenital deficiency of alpha 1-antitrypsin with liver involvement were identified on clinical grounds; 10 cases of liver cirrhosis were selected by way of the diastase-PAS-reaction. In these 20 cases alpha 1-antitrypsin was demonstrated in paraffin sections by a modification of the indirect peroxidase-antiperoxidase method. In all these cases the immunoreactive inclusions could be demonstrated in the hepatocytes, even when the paraffin sections were several years old. 399 cases of cirrhosis of the liver were investigated in this retrospective study; only 10 of these cases had, as mentioned above, inclusions in the hepatocytes, which were immunoreactive, PAS-positive, and resistant against diastase.
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