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  • Title: Evaluation of a clostridial alpha-toxin disk test for rapid presumptive identification of group B streptococci.
    Author: Smith JA, Ngui-Yen JH.
    Journal: J Clin Microbiol; 1980 Jul; 12(1):18-21. PubMed ID: 6252240.
    Abstract:
    An alpha-toxin disk test is described in which group B streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of Clostridium perfringens. The test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. A total of 95% of strains of pure group B streptococci tested produced positive reactions within 5 h, and all were positive after overnight incubation, with patterns of synergistic hemolysis readily distinguishable from those seen with group A streptococci. The preparation of disks is well within the scope of most clinical laboratories.
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