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  • Title: Nucleolar ultrastructure and transcription of preribosomal RNA in Rous sarcoma virus-transformed chick embryo fibroblasts treated with alpha-amanitin.
    Author: Connan G, Mongiat F, Toulemont Y, Haguenau F, Rabotti F.
    Journal: J Natl Cancer Inst; 1980 Dec; 65(6):1245-56. PubMed ID: 6253713.
    Abstract:
    Uninfected and Rous sarcoma virus (RSV)-transformed chick embryo fibroblasts (CEF) were exposed to various concentrations of alpha-amanitin for different lengths of time. At a concentration of 4 micrograms alpha-amanitin/ml, RSV-transformed cells were shown to maintain a normal rate of transcription of all classes of RNA, whereas in uninfected cells transcription was reduced to a very low level. These observations cannot be accounted for by a difference in the penetration rate of alpha-amanitin through the plasma membrane. Investigation of the ultrastructure showed that the degree of nucleolar fragmentation induced by alpha-amanitin was comparable in both types of cells. Persistence of transcription with concomitant nucleolar fragmentation in alpha-amanitin-treated RSV-transformed CEF is not in accord with the hypothesis that nucleolar integrity is required for preribosomal RNA transcription.
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