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  • Title: Origin of DNA replication of bacteriophage f1 as the signal for termination.
    Author: Horiuchi K.
    Journal: Proc Natl Acad Sci U S A; 1980 Sep; 77(9):5226-9. PubMed ID: 6254068.
    Abstract:
    Restriction fragments that contain the origin of DNA replication of bacteriophage f1 were inserted in vitro into circular f1 DNA molecules to form genomes that contain two origins. This DNA was used to transfect Escherichia coli. Analyses of the DNA of the progeny phage indicated that one origin and the DNA segment located between the two origins in the infecting DNA molecules had been eliminated. This result is interpreted to mean that the nucleotide sequence of the origin for plus (viral)-strand synthesis also serves as the signal for the termination of DNA synthesis.
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