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  • Title: Syntheses of some fluorescent ribonucleotides.
    Author: Tsou KC, Yip KF.
    Journal: Nucleic Acids Res; 1980 Feb 11; 8(3):567-72. PubMed ID: 6255445.
    Abstract:
    Synthesis of guanosine 3'-phosphodiesters of 1, N6-ethenoadenosine-2-sulfonate or 1, N6-etheno-2-azaadenosine can be done readily with ribonuclease N1. There is a 10-fold difference in quantum yield between the phosphodiesters and the starting fluorescent nucleosides. This difference provides the basis for the development of a fluorescent assay for nuclease.
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