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  • Title: Detection of pseudorabies virus antibodies: time of appearance by two serotests.
    Author: Haffer K, Gustafson DP, Kanitz CL.
    Journal: Am J Vet Res; 1980 Aug; 41(8):1317-8. PubMed ID: 6255835.
    Abstract:
    This report describes a time-course comparison of detection of pseudorabies virus antibodies in experimentally infected swine by the virus-neutralization (VN) and indirect hemagglutination tests. Specific antibody titers were observed by the IHA test at 5 days after swine were inoculated, but not until 12 days by the VN test. The predominant immunoglobulin (Ig) class present in the serums of the swine at 5 and 7 days after inoculation was IgM, as determined by sulfhydryl reductions. The VN test lacked sensitivity to early Ig levels (IgM) in these experimentally infected swine, while the indirect hemagglutination test was highly sensitive to these same levels. On the basis of these results, it is possible that the VN test may read early infections as pseudorabies virus negative, due to the low presence of IgG in these samples.
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