These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Ectoenzymes on the surface of cells from human lymphoblastoid lines: 5'-nucleotidase and phosphatase.
    Author: Gutensohn W, Gürtler LG, Siegert W, Eichler E, Ernst S.
    Journal: Blut; 1980 Dec; 41(6):411-20. PubMed ID: 6256029.
    Abstract:
    Activities of the ecto-enzymes 5'-nucleotidase (5'-N) and phosphatase were determined on the surface of intact cells from 15 different established lines of human B- and T-lymphoblasts. Whereas all the lines express phosphatase, 10 of the lines were negative for 5'-N. 5'-N-negative cell lines are found among B as well as T cells, and they do not carry cryptic enzyme activity. In a 5'-N-positive line activity of this enzyme is correlated with growth showing a peak during the logarithmic phase. On the other hand, inhibition of 5'-N does not change the growth curve of this line. Neuraminidase treatment of the cell surface brings about an increase in phosphatase but not in 5'-N activity. 5'-N of two B-cell lines of human peripheral blood lymphocytes shows complete crossreactivity with an antiserum obtained against human placental 5'-N. However, the enzyme of one lymphoma line with B-cell properties (EHR-A-Ramos) does not cross-react with this serum. The results are discussed with respect to suitability of these lymphoblast lines as model systems for the study of immunodeficiencies.
    [Abstract] [Full Text] [Related] [New Search]