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  • Title: [Isolation and radiometric determination of rat liver ATP: thiamine diphosphate phosphotransferase activity].
    Author: Voskoboev AI, Luchko VS.
    Journal: Vopr Med Khim; 1980; 26(4):564-8. PubMed ID: 6256962.
    Abstract:
    A radioassay was developed for estimation of the thiamin diphosphate kinase activity (EC 2.7.4.15); 14C-TDP was used as a substrate. After termination of the reaction (formation of TTP), the products obtained were separated using ion exchange chromatography on SP-Sephadex C-25. The method developed was very sensitive and enabled to estimate the enzymatic activity in tissue homogenates. TDP-kinase was isolated from rat liver with a 70-fold purification. Dependence of the reaction rate on pH value, concentrations of the enzyme and thiamin were studied.
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