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  • Title: Detection of infectious centers in C57BL/Ka lymphoid cell populations infected in vitro by the radiation leukemia virus.
    Author: Boniver J, Declève A, Finn OJ, Honsik C, Lieberman M, Kaplan HS.
    Journal: Cancer Res; 1980 Mar; 40(3):544-9. PubMed ID: 6258781.
    Abstract:
    The cocultivation of nonproducer lymphoma cells derived from a radiation-induced lymphoma of the C57BL/Ka mouse with cultures of lymphoid cell populations from the thymus, spleen, and marrow of the same strain 48 hr after their infection by the C57BL/Ka leukemia viruses permits the detection of infectious centers in these cultures. A quantitative assay is described which allows the estimation in lymphoid cell subpopulations of the numbers of target cells susceptible to productive infection by the thymotropic and leukemogenic viruses of C57BL/Ka mice in vitro. This assay should greatly facilitate the identification and characterization of such target cells.
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