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  • Title: Effect of polyamines on purified poly(ADP-ribose) synthetase from rat liver nuclei.
    Author: Kawamura M, Tanigawa Y, Kitamura A, Miyake Y, Shimoyama M.
    Journal: Biochim Biophys Acta; 1981 Jan 29; 652(1):121-8. PubMed ID: 6260184.
    Abstract:
    Using the purified enzyme system, we investigated the spermine-induced stimulation of poly(ADP-ribose) formation. In the presence of 2 microgram DNA and 10 mM Mg2+ in a reaction mixture of 0.2 ml, the optimal concentrations of spermine, spermidine and putrescine were 10, 12.5 and 35 mM, respectively. With the omission of Mg2+, there was an increase in optimal concentrations of polyamines without changes in the rate of poly(ADP-ribose) formation. The concentrations of spermine required for maximum synthesis of poly(ADP-ribose) were increased in proportion to the increase in the amount of DNA. Kinetic analysis of poly(ADP-ribose) formation showed a decrease in Km values for NAD with the addition of spermine. The possible relationship of polyions with regard to DNA, spermine and histone in the purified enzyme system was discussed.
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