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  • Title: Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli.
    Author: Palva ET, Hirst TR, Hardy SJ, Holmgren J, Randall L.
    Journal: J Bacteriol; 1981 Apr; 146(1):325-30. PubMed ID: 6260742.
    Abstract:
    Escherichia coli K-12 minicells were employed to investigate the biosynthesis of plasmid-encoded, heat-labile enterotoxin of E. coli. Two polypeptide species related to the B subunit of the toxin were expressed in the minicells. One of these polypeptides (molecular weight, 11,500) was immunoprecipitated by antiserum to cholera toxin. Because the B subunits of heat-labile enterotoxin and cholera toxin have common antigenic sites, we concluded that this species was the mature B subunit. The larger polypeptide (molecular weight, 13,000) is likely to be a precursor of the B subunit because it could be chased into the mature form. This conversion was inhibited by compounds which dissipate proton motive force, suggesting that processing requires energy.
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