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  • Title: Subcellular location of two enzymes involved in the synthesis of phosphorylated recognition markers in lysosomal enzymes.
    Author: Waheed A, Pohlmann R, Hasilik A, von Figura K.
    Journal: J Biol Chem; 1981 May 10; 256(9):4150-2. PubMed ID: 6260788.
    Abstract:
    Phosphorylated recognition markers in lysosomal enzyme appear to be synthesized by transfer of alpha-N-acetylglucosamine 1-phosphate groups to C6 hydroxyl of mannose residues in glycosylated enzyme precursors and a subsequent hydrolysis from the diester groups of the N-acetylglucosamine residues. The transfer and the diesterase activities were studied in subcellular fractions of rat liver. Both activities fractionated like the Golgi marker galactosyltransferase.
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