These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [(Na+, K+)-dependent adenosine triphosphatase in the rat brain, heart and kidney. Changes in the enzymatic metabolic quotient in purification with sodium deoxycholate].
    Author: Konstantinov B.
    Journal: Eksp Med Morfol; 1981; 20(1):18-21. PubMed ID: 6262044.
    Abstract:
    The usage of detergents is included in most of the proposed methods for purification of a membrane bound enzyme (Na+, K+)-dependent adenosine triphosphatase (EK 3.6.1.3). The detergents influence the lipid envelope of an enzyme, which is considered as an integral part of the enzymic molecule, and extract nonactive proteins from the membrane. The aim of the work is measurement of the metabolic number of the enzyme after two-fold treatment with ionic detergent-sodium desoxycholate in brain, renal and heart tissue of a rat. He finds that the desoxycholate alters the metabolic number in the preparations from renal and brain tissue and does not change it in the heart tissue. These differences according to the author are due to differences in the membrane structures of the tissues. The usage of two-fold treatment of enzyme preparations with sodium desoxycholate is unsuitable for purification of (Na+, K+)-dependent adenosine triphosphatase in kidneys and brain.
    [Abstract] [Full Text] [Related] [New Search]