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  • Title: Characterization and determination of neuropeptides by high-performance liquid chromatography and radioimmunoassay.
    Author: McDermott JR, Smith AI, Biggins JA, Al-Noaemi MC, Edwardson JA.
    Journal: J Chromatogr; 1981 Mar 13; 222(3):371-9. PubMed ID: 6262333.
    Abstract:
    A method is described for the separation and analysis of a variety of neuropeptides using reversed-phase high-performance liquid chromatography coupled with radioimmunoassay. The solvent system (an acetonitrile gradient containing 0.08% trifluoroacetic acid) allows UV detection at 206 nm, gives good resolution and, by being volatile, is readily compatible with radioimmunoassay. Three applications of the method are described: (a) thyrotropin releasing hormone immunoreactivity in the rat brain has been characterized; (b) ACTH immunoreactivity in the rat pituitary pars intermedia has been resolved into its component peptides; (c) degradation of luteinizing hormone releasing hormone in vitro has been followed.
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