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Title: Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid:deoxyribonucleic acid hybridization. Author: Brautigam AR, Richman DD, Oxman MN. Journal: J Clin Microbiol; 1980 Aug; 12(2):226-34. PubMed ID: 6262368. Abstract: A method for typing clinical isolates of herpes simplex virus was developed. It utilizes hybridization between unlabeled deoxyribonucleic acid from infected cultures and tritium-labeled virus deoxyribonucleic acid, and it can be completed within a day using a single roller-tube culture of the clinical isolated. The data obtained are inherently quantitative, and the method yields unequivocal identification and typing. Thirty-nine coded clinical isolates were all correctly typed by this method.[Abstract] [Full Text] [Related] [New Search]