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  • Title: Isolation of identification of 24(R)-hydroxyvitamin D3 from chicks give large doses of vitamin D3.
    Author: Wichmann J, Schnoes HK, DeLuca HF.
    Journal: Biochemistry; 1981 Apr 14; 20(8):2350-3. PubMed ID: 6263312.
    Abstract:
    A new metabolite of vitamin D was isolated from the blood plasma of chicks given large doses of vitamin D3. The isolation involved methanol-chloroform extraction and four column chromatographic steps. The metabolite was identified by high- and low-resolution mass spectroscopy, chemical derivatization, an comigration with authentic standard as 3 beta, 24(R)-dihydroxy-9,10-seco-5,7,10(19)-cholestatriene [24(R)-hydroxyvitamin D3]. No detectable 24-(R)-hydroxyvitamin D3 was recovered from 16 L of plasma from chicks receiving physiologic levels of vitamin D3.
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