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  • Title: [Isolation of NAD-kinase from pigeon heart].
    Author: Bulygina ER, Telepneva VI.
    Journal: Biokhimiia; 1980 Nov; 45(11):2019-27. PubMed ID: 6263365.
    Abstract:
    A procedure of isolation of pigeon breast muscle NAD-kinase resulting in a 100--130-fold purification of the enzyme with a total yield of 30--35% is described. The enzyme is electrophoretically homogenous; its molecular weight as determined by SDS-electrophoresis is 45 000. The partially purified preparation contains multiple enzymic forms with molecular weights varying from 45 000 to 270 000, which represent an equilibrious system of structurally different oligomers. At the last purification step, i. e. ion-exchange chromatography, the enzyme loses its ability for oligomerization. Possible causes of disappearance of the enzyme multiple forms during purification are discussed.
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