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  • Title: Adenosine 3',5'-monophosphate (cAMP)-binding proteins and cAMP-dependent protein kinases in bovine adrenal cortical cell plasma membrane.
    Author: Reitherman R, Chen L, Wen S, Harding BW.
    Journal: Endocrinology; 1981 Jul; 109(1):301-6. PubMed ID: 6263589.
    Abstract:
    A cAMP-binding protein and cAMP-dependent protein kinase have been identified and partially characterized in bovine adrenocortical plasma membranes. [3H]cAMP binding to the plasma membrane preparation demonstrates both high (Ka = 4.2 X 10(9) M-1) and low (Ka = 1.3 X 10(8) M-1) affinity binding sites. cAMP-dependent protein kinase was demonstrated using both bovine histone and endogenous substrates. The detergent-dependent solubilization characteristics of this cAMP-dependent protein kinase indicate that it is an integral membrane protein.
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