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  • Title: SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.
    Author: Chu G, Sharp PA.
    Journal: Gene; 1981 Mar; 13(2):197-202. PubMed ID: 6263759.
    Abstract:
    A modification of the Graham and Van der Eb (1974) DNA-calcium phosphate coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA. The transfection is done in suspension after detachment of cells by trypsin digestion. Transfection efficiency was measured by staining cells for the presence of SV40 T-antigen by indirect immunofluorescence and by assaying for the presence of SV40 early message by the Berk and Sharp (1978) technique.
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