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  • Title: [Investigation of radiologic lung fine structure by freezing of inflated specimens in liquid nitrogen (author's transl)].
    Author: Rau WS, Hauenstein K, Volk P, Mittermayer C.
    Journal: Rofo; 1980 Oct; 133(4):400-5. PubMed ID: 6263768.
    Abstract:
    Radiologic pathologic correlation of lung specimens requires a gas content identical to intravital conditions. Methods of air drying and formalin steam fixation are well known but time consuming. A new, quick method is presented: The lungs are inflated with compressed air, frozen in liquid nitrogen, and cut into slices. Radiographs taken in soft tissue technique will demonstrate structural details down to 200--300 mu. The excellent state of preservation enables unusual quality of microscopic slides, as well.
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