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  • Title: The effect of aging on beta-adrenoceptor-stimulated cyclic AMP formation in human lymphocytes.
    Author: Kraft CA, Castleden CM.
    Journal: Clin Sci (Lond); 1981 May; 60(5):587-9. PubMed ID: 6265139.
    Abstract:
    1. Responsiveness of the beta-adrenoceptor adenylate cyclase system was measured in lymphocytes from healthy young and old subjects by incubating the cells with isoprenaline in the presence of a phosphodiesterase inhibitor and by measuring production of adenosine 3':5'-cyclic monophosphate (cyclic AMP) with a competitive binding assay. 2. The two groups did not differ significantly in the levels of cyclic AMP produced or in the concentration of isoprenaline required to give half-maximal stimulation of the cells (ED50).
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